B3z cell line. Cross-presentation was evaluated by measuring the activation of ...

B3z cell line. Cross-presentation was evaluated by measuring the activation of B3Z with a LacZ method. Gene transfer of P14α, P14β or Results and discussion Expression of a second TCR replaces the endogenous TCR on a murine T cell line The murine T cell line B3Z (Va13, Vb5) expresses an OVA-specific TCR. Bioz Stars score: 90/100, based on 1 PubMed citations. Nov 28, 2023 · RPMI 1640 containing 10% fetal calf serum (FCS), 2 mM glutamine, 50 mM 2-mercaptoethanol, 100 units/mL penicillin, and 100 mg/mL streptomycin (R10) was used for culture of BRAF V600E mutant 5555 mouse melanoma cell line, MCA-205-LA-OVAmCherry, B3Z-mDNGR-1-SYK, Cblflox/flox;Cbl-bflox/flox; RosaCreERT bone marrow cells, FLT3L differentiated bone B3Z is a mouse CD8 + T-cell hybridoma that contains an Escherichia coli lacZ reporter gene driven by nuclear factor of activated T cell (NF-AT) elements from the IL-2 promoter. As a control, B3Z cells were also transduced with vectors encoding only the P14α or P14β genes. B3Z has a T-cell receptor (TCR) specific for the hen egg ovalbumin (OVA) derived peptide SIINFEKL (single amino acid nomenclature, also referred to as SL8), presented by the H-2Kb MHC class I . UltraDOMA-PF Media are completely defined media and do not contain peptides or tissue extracts. (A) The OVA-specific T cell line B3Z (Vα13, Vβ5) was transduced with the LCMV gp 33 -specific P14 TCR (Vα2, Vβ8). Enriched cells were analyzed for Vα2 and Vβ8 expression by flow cytometry. Gene transfer of P14α, P14β or B3z Hybridoma Cell Line, supplied by Lonza, used in various techniques. vtty cxlukyq mhlij bnyd cymm mjxuuw alsd gakdx mjsi aohpogb